蛋白质精氨酸甲基转移酶1调控DNA损伤修复和细胞凋亡     

张一超  吴韦韦  李奥  刘宝华  庞秋香 《海洋科学》2020,44(3):146-152

蛋白质精氨酸甲基转移酶1 (protein arginine methyltransferases 1, PRMT1)是PRMT家族中的重要一员,属于Ⅰ型PRMTs,细胞内超过85%的蛋白质精氨酸的甲基化由其催化。PRMT1通过调节底物的甲基化水平,从而调控蛋白的功能及蛋白参与的细胞活动和生理过程,包括细胞信号转导,DNA损伤修复,转录调控,RNA代谢,蛋白质相互作用等。本文着重讨论PRMT1的结构、底物及其在DNA损伤修复和细胞凋亡中的功能。  相似文献   

不同温度条件下褐菖鲇幼鱼的耗氧率和排氨率     

邱成功  徐善良  林少珍  程亮  刘飞 《台湾海峡》2014,(1):84-89

利用静水密闭式呼吸仪,测定了不同温度（10、12、……、30℃）条件下褐菖鼬（Sebastiscus marmoratus）幼鱼耗氧率和排氨率变化．结果表明,温度对幼鱼耗氧率和排氨率均有显著影响（P〈0．05）．温度10～30℃,幼鱼耗氧率、排氨率变化范围分别为0．02～0．30mg／（g·h）和2．64～10．01ug／（g·h）,温度26、16℃时耗氧率和排氨率分别上升至峰值,分别为最小值10℃组的15．00、3．79倍;温度（F）对幼鱼耗氧率R0[mg／（g·h）]和排氨率RN[ug／（g·h）]的影响可分别用多项式表示：R0=-2．00×10^-5 T4＋1．50×10^-3 T3-3．69×10^-2 T2＋0．3978T-1．5376,R2=0．988和RN=3．00×10^-5 T6 -4．00×10^-3 T5＋0．1996 T4-5．1111T3＋70．817T2-501．10T＋1415．80,R2=0．964;幼鱼的氨熵变化范围为0．02～0．19,其蛋白质供能比变化范围为5．64％～56．65％,平均蛋白质供能比为22．56％;各温度跨度代谢率Q10值均值为4．02,18～28℃代谢率Q10值为2．81±0．09与鱼类平均Q10值较接近;各温度跨度组排泄率Q10均值为0．93,对比同一温度跨度组代谢率和排泄率Q10值,代谢率Q10值均大于排泄率Q10值两倍以上,最大组间相差达7倍．因此,幼鱼能量消耗的供能物质以脂肪和碳水化合物为主,蛋白质为辅．此外,温度变化对幼鱼代谢的影响程度明显大于对排泄的影响,且其适宜生长温度为18～28℃．  相似文献   

饲料蛋白质水平对大黄鱼体内蛋白质的沉积和代谢反应的影响     

林淑琴  成琳  张文兵  徐玮  麦康森  申屠基康 《中国海洋大学学报(自然科学版)》2014,44(5):32-39

进行9周的养殖实验,比较分析饲料蛋白质水平对3个不同生长阶段大黄鱼体内蛋白质沉积和代谢反应相关指标的影响。实验用大黄鱼分为3种规格,分别是小规格鱼(SF,初始体重:(12.80±0.08)g)、中规格鱼(MF,初始体重:(137.90±0.81)g)和大规格鱼(LF,初始体重:(194.10±2.37)g)。饲料中的7个蛋白质梯度设置为30.4%,34.2%,38.5%,42.2%,46.0%,50.3%和54.3%。结果表明,随着饲料蛋白质水平的上升,SF的蛋白质含量和氮保留率显著升高(P0.05),而MF和LF的全鱼蛋白质含量(BP)和氮保留率(NR)未见显著变化(P0.05)。从不同生长阶段来看,氮保留率MF显著低于SF和LF(P0.05)。3个生长阶段,大黄鱼肝脏中的谷草转氨酶(AST)和谷丙转氨酶(ALT)活性,以及血清中的血氨、尿素氮和总氨基酸含量均随着饲料蛋白质水平的上升而显著升高(P0.05)。从不同生长阶段来看,血氨含量MF显著高于SF和LF(P0.05)。研究表明,大黄鱼体内蛋白质沉积和氨基酸代谢随饲料蛋白质水平的上升而增强,大黄鱼的氮保留能力MF阶段低于SF和LF阶段。  相似文献   

不同蛋白水平饲料对刺参生长和消化酶活性的影响   总被引：1，自引：0，他引：1  

冯丹  高勤峰  董双林 《中国海洋大学学报(自然科学版)》2014,44(2)

通过在刺参饲料中添加不同含量的鱼粉,调节饲料中蛋白水平,测定不同蛋白水平对刺参(Apostichopus japonicus)消化酶活性的影响,以稳定同位素分析技术研究刺参对不同食物组分的吸收效率。实验分6个饲料处理组,鱼粉添加量分别为0(A组)、2%(B组)、5%(C组)、10%(D组)、15%(E组)和20%(F组)。实验过程中,分别于初始时间、第一周、第二周、第四周、第八周和第十二周采集刺参样品,测定刺参的特定生长率、稳定同位素13 C/12 C、15 N/14 N值和消化酶活性。结果显示:D组刺参的特定生长率最高,且与C组无显著差异(P0.05),F组显著低于其它组(P0.05);D组鱼粉对刺参的食物贡献率最高,显著高于其它组(P0.05);消化酶活性方面,蛋白酶和淀粉酶活性均为C组最高,且在饲养过程中,蛋白酶活性一直呈上升趋势,而淀粉酶活性则是先下降后上升。从刺参生长、鱼粉食物贡献率和消化酶活性综合考虑,鱼粉添加量在5%~10%最利于刺参生长。  相似文献   

Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales,Phaeophyta)     

邵展茹  刘福利  李秋莹  姚建亭  段德麟 《中国海洋湖沼学报》2014,32(2):377-389

Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments,and consequently may have highly effi cient ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)activity for carbon assimilation.In our study,we cloned the full-length Rubisco gene from S.japonica(SJ-rbc).It contained an open reading frame for a large subunit gene(SJ-rbcL)of 1 467 bp,a small subunit gene(SJ-rbcS)of 420 bp,and a SJ-rbcL /S intergenic spacer of 269 bp.The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa,5.81 and 15.84 kDa,4.71,respectively.After induction with 1 mmol/L isopropyl-β-Dthiogalactopyranoside for 5 h and purifi cation by Ni 2+ affi nity chromatography,electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein.Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night.This observation implied that Rubisco played a role in normal gametophytic growth and development.In juvenile sporophytes,mRNA levels of SJ-rbcL,carbonic anhydrase,Calvin-BensonBassham cycle-related enzyme,and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance.Similarly,expression of these genes was up-regulated under blue light irradiance at 350 μmol/(m 2·s).Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements.  相似文献   

水流速率对全缘马尾藻幼孢子体生长和生理活性的影响     

黄苑媚  ;刘志刚  ;谢恩义  ;刘付少梅  ;孙宗红 《广东海洋大学学报》2014,(6):45-50

在0、0.5、1、2、4、8 cm/s 等5 种不同水流速率下培养全缘马尾藻（Sargassum integerrimum）幼孢子体,研究水流速率对幼孢子体质量、叶绿素a 含量、SOD 活力、CAT 活力和蛋白质浓度等生理指标的影响.结果表明,以1、2、4 cm/s 流速的幼孢子体质量增长最快,3 种流速的增长差异不具统计学意义（P〉0.05）.在生理指标方面：叶绿素a 含量以1、2、4 cm/s 流速最高,3 种流速的含量差异不具统计学意义（P〉0.05）;SOD 比活性以流速0.5、1、2 cm/s 的活力最低,各组间差异不具统计学意义（P〉0.05）;CAT 比活性以流速1、2 cm/s 的活力最低,组间差异不具统计学意义（P〉0.05）;蛋白质含量以1、2、4 cm/s 流速最大,组间差异具统计学意义（P〈0.05）.水流速率对体质量增长和各生化指标均产生显著的影响,以流速1～4 cm/s 培育全缘马尾藻培幼孢子体较合适.  相似文献   

草鱼(Ctenopharyngodon idellus)呼肠孤病毒(GCRV)VP6相互作用蛋白的筛选     

丁燏  李杰  闫秀英  简纪常  吴灶和 《海洋与湖沼》2014,45(6):1295-1299

为筛选与草鱼呼肠孤病毒GCRV096 VP6发生相互作用的宿主蛋白,先将VP6基因的PCR扩增产物,克隆至酵母表达载体p GBKT7以构建其诱饵质粒(p GBKT7-VP6);再将酵母菌Y2HGold(含p GBKT7-VP6)与酵母菌Y187[含草鱼肾脏细胞(CIK)c DNA文库质粒]进行人工诱导融合,然后经选择培养筛选阳性克隆。结果共筛选到4株阳性克隆,经测序、生物信息学分析,分别属于两条不同的核苷酸序列,其中之一所编码的产物与GAPDH(3-磷酸甘油醛脱氢酶)同源性较高。可见,该酶极可能在GCRV096侵染宿主的初期发挥着重要作用。  相似文献   

废弃鱼骨中蛋白质的提取分离及酶解工艺研究     

张振兴  刘永峰  刘毅  裴栋  魏鉴腾  邸多隆 《海洋科学》2014,38(6):31-36

以蛋白提取率和多肽分子质量分布为评价指标,采用不同提取方法和不同蛋白酶对废弃鱼骨蛋白质的提取和酶解工艺进行了研究。结果表明,高压蒸煮法提取的鱼骨蛋白质中蛋白含量为86.15%,蛋白提取率为16.76%,明显高于恒温水浴和热回流提取法。中性蛋白酶酶解的多肽中蛋白含量为88.46%,多肽比例高达95%以上,明显高于其他三种酶。先利用高压提取法提取蛋白质,再利用中性蛋白酶酶解蛋白质制备多肽,可降低酶用量,该方法为多肽工业化生产提供参考及新思路。  相似文献   

Comparative study on the allergenicity of different Litopenaeus vannamei extract solutions   总被引：1，自引：0，他引：1  

WU Lisha  LIN Haixin  WANG Guoying  LU Zongchao  CHEN Guanzhi  LIN Hong  and LI Zhenxing 《中国海洋大学学报(英文版)》2014,13(1):157-162

Allergen extracts are widely used for allergy diagnosis and treatment. The application of shrimp extract is hampered due to the low protein concentration and the inconsistent allergenicity. Extracting solutions are considered to be the primary limiting factor of protein extraction from crustaceans. This study aimed to select an optimal solution for shrimp protein extraction by comparing the allergenicity of different shrimp extracts. The effect of 7 existing or modified extracting solutions were evaluated, including the glycerol-NaCl solution, the glycerol Cocaine's solution, the buffered saline solution, the Cocaine's solution, the Glucose leaching solution, 1 mol L-1 KCl solution, and 0.01 mol L-1 phosphate buffered saline solution with and without dithiothreitolor(DTT). The quantitative(protein concentration) and qualitative parameters(SDS-PAGE protein patterns and immuno-reactivity) were determined using the sodium dodecyl sulfate polyacrylamide gel electrophoresis, enzyme linked immunosorbent assay and immunoblotting assay. Results showed that the 1 mol L-1 KCl solution with DTT was optimal for shrimp protein extraction, which yielded high concentration and allergenicity in the protein extract, including major and minor allergens. The 1 mol L-1 KCl solution with DDT is proposed for preparation of shrimp extract and associated allergy diagnosis, as well as potential applications for other crustaceans.  相似文献   

Effect of dissolved oxygen on swimming ability and physiological response to swimming fatigue of whiteleg shrimp (Litopenaeus vannamei)     

DUAN Yan  ZHANG Xiumei  LIU Xuxu  and Dhanrajsingh N. Thakur 《中国海洋大学学报(英文版)》2014,13(1):132-140

The swimming endurance of whiteleg shrimp(Litopenaeus vannamei, 87.66 mm ± 0.25 mm, 7.73 g ± 0.06 g) was examined at various concentrations of dissolved oxygen(DO, 1.9, 3.8, 6.8 and 13.6 mg L-1) in a swimming channel against one of the five flow velocities(v1, v2, v3, v4 and v5). Metabolite contents in the plasma, hepatopancreas and pleopods muscle of the shrimp were quantified before and after swimming fatigue. The results revealed that the swimming speed and DO concentration were significant factors that affected the swimming endurance of L. vannamei. The relationship between swimming endurance and swimming speed at various DO concentrations can be described by the power model(ν·tb = a). The relationship between DO concentration(mg L-1) and the swimming ability index(SA∫ 9000I), defined as SAI =vdt( cm), can be described as SAI = 27.947 DO0.137(R2 = 0.9312). The 0level of DO concentration directly affected the physiology of shrimp, and exposure to low concentrations of DO led to the increases in lactate and energetic substrate content in the shrimp. In responding to the low DO concentration at 1.9 mg L-1 and the swimming stress, L. vannamei exhibited a mix of aerobic and anaerobic metabolism to satisfy the energetic demand, mainly characterized by the utilization of total protein and glycogen and the production of lactate and glucose. Fatigue from swimming led to severe loss of plasma triglyceride at v1, v2, and v3 with 1.9 mg L-1 DO, and at v-11 with 3.8, 6.8 and 13.6 mg L DO, whereas the plasma glucose content increased significantly at v3, v4 and v5 with 3.8 and 6.8 mg L-1 DO, and at v5 with 13.6 mg L-1 DO. The plasma total protein and hepatopancreas glycogen were highly depleted in shrimp by swimming fatigue at various DO concentrations, whereas the plasma lactate accumulated at high levels after swimming fatigue at different velocities. These results were of particular value to understanding the locomotory ability of whiteleg shrimp and its physiological changes, further contributing to the improvement of capture and rearing technique.  相似文献